大连理工大学学报Issue(3)：340-345,6.
两相体系中固定化联苯双加氧酶全细胞产靛蓝特性研究
Research on characteristics of biotransformation of indigo by immobilized whole cell expressing biphenyl dioxygenase in biphasic system
摘要
Abstract
The extraction of indigo is delayed due to the adsorption of indigo by the free whole cell , which is one of the major difficulties during the biotransformation of indigo .Three immobilization methods are used , and sodium alginate immobilization method obtains the best results . The immobilized cell can be repeatedly used for two times in a biphasic system under the optimum immobilization condition :3 .5% sodium alginate ,1% CaCl2 ,and the indigo production is 15 .9 mg/L after 6 h of the first transformation .Besides ,the indigo decomposition can be avoided by the biphasic system with 40% dodecane as the optimal organic phase .The optimum indole tolerance concentration is 400 mg/L ,and the productivity of indigo is 0 .28 mg/mg .关键词
联苯双加氧酶/基因工程菌/靛蓝/海藻酸钠/两相体系Key words
biphenyl dioxygenase/gene engineering bacteria/indigo/sodium alginate/biphasic system分类
生物科学引用本文复制引用
曲媛媛,许炳雯,丛培,吴英格,朱丹,马桥,张旭旺,周集体..两相体系中固定化联苯双加氧酶全细胞产靛蓝特性研究[J].大连理工大学学报,2013,(3):340-345,6.基金项目
国家自然科学基金资助项目（21176040，51078054）． （）
