动物医学进展Issue(11)：25-30,6.
犊牛睾丸生殖干细胞与支持细胞体外共培养体系的建立
Establishment of Co-culture System for Calf Testis Germ Stem Cells and Sertoli Cells in Vitro
摘要
Abstract
This experiment was conducted to establish the co-culture system of calf mGSCs and SCs,and compare the effect of Sertoli cells (SCs)density on calf testis germ stem cells (GSCs)growth.We separa-ted and identified mGSCs and SCs,covered GSCs on different density SCs,and then observed cell morphol-ogy,counted the colony of the GSCs and measured the relative expressions of the two cells specific genes. The results showed that the calf mGSCs have the same characteristics with mouse mGSCs,AKP strain was positive,the co-culture cells expreessed SCs′sign gene SCF and mGSCs′sign gene OCT-4;the effect of cul-ruted mGSCs of low-density SCs(225/cm2 )was better than that of medium-density SCs (1 300/cm2 )and high-density SCs (5 600/cm2 ):The GSC colony purity and volume were the best.The GSC colony pro-portion increased significantly than that in medium-density SCs cultures (P <0.05),increased very signifi-cantly than that in high-density SCs cultures (P <0.01).The qRT-PCR result showed that compared with medium-density and high-density SCs,low-density SCs made the GSC clones grow significantly well with expressions of OCT-4/SCF increased very significantly (P <0.01).These results indicated that the excel-lent testis GSCs can be acquired by low-density SCs when establishing the co-culture system of calf mGSCs and SCs.关键词
睾丸支持细胞/睾丸生殖干细胞/共培养/密度/集落Key words
Sertoli cell/testis germ stem cell/co-culture/density/colony分类
农业科技引用本文复制引用
张倩,齐晓楠,杨文浩,丛霞,李华涛,曹荣峰,田文儒..犊牛睾丸生殖干细胞与支持细胞体外共培养体系的建立[J].动物医学进展,2015,(11):25-30,6.基金项目
国家自然科学基金项目（31172378）；山东省现代农业产业技术体系团队项目（SDAIT-12-011-03）；青岛农业大学研究生创新项目 （）
