动物医学进展Issue(11)：36-39,4.
奶牛乳房炎致病菌的分离鉴定及耐药性研究
Study on Isolation,Identification and Drug Resistance of Pathogenic Bacteria of Dairy Cow Mastitis
摘要
Abstract
In order to undersand microflora distribution of pathogenic bacteria of dairy cow mastitis,as well as path-ogenicity and drug resistance of opportunistic pathogens.302 milk samples of clinical mastitis were collected,from which 257 bacterial strains were isolated and identified by 16 S DNA PCR kit.The tests on mouse pathogenicity and drug resistance were performed for the opportunistic pathogens with high ratios.The results showed that de-tected bacteria could be classified as main pathogens,opportunistic pathogens,lactic acid bacteria and other bacte-ria.Opportunistic pathogens,in which A.pyogenes had obvious pathogennicity in mice,E.faecalis and E.fae-cium did not,but they had certain resistance to antimicrobials.The E.faecalis and E.fecium with a pathogenic condition,belong to lactic acid bacteria that are generally used as probiotics.It showed the pathogenic bacteria of dairy cow mastitis had diversity and complexity.In addition to main pathogens and opportunistic pathogens,some probiotics getting into breast tissue might become opportunistic pathogens to cause mastitis.关键词
奶牛乳房炎/致病菌/致病性/耐药性Key words
Dairy cow mastitis/pathogenic bacteria/pathogenicity/drug resistance分类
农业科技引用本文复制引用
李新圃,李宏胜,罗金印,杨峰,王旭荣..奶牛乳房炎致病菌的分离鉴定及耐药性研究[J].动物医学进展,2015,(11):36-39,4.基金项目
“十二五”国家科技支撑计划（2012BAD12B03）；甘肃省自然科学基金项目（1308RJZA119）；甘肃省农业科技创新项目（GNCX-2013-59）；甘肃省科技支撑计划 （）
