动物医学进展Issue(11)：44-48,5.
托氟沙星纳米乳的体内外抗菌作用研究
In Vitro and in Vivo Antibacterial Activities of Tosufloxacin Nanoemulsion
摘要
Abstract
The aim of this study was to investigate the antibacterial activity in vitro and pharmaceutical effect in vi-vo of tosufloxacin nanoemulsion.Microtiter broth dilution method recommended by Clinical and Laboratory Stand-ards Institute (CLSI)was adopted to determine the MIC and MBC of tosufloxacin nanoemulsion and compared with that of levofloxacin hydrochloride;the pharmaceutical effect was evaluated by oral administration to the model chicks successfully infected with Salmonella typhimurium containing different concentrations of tosufloxacin nano-emulsion.The results showed the MICs of tosufloxacin nanoemulsion against Streptococcus agalactiae ,Staphylo-coccus aureus ,Pasteurella multocida,Escherichia coli,Shigella dysenteriae ,Salmonella typhimurium,Salmonella paratyphi-A were 0.25 μg/mL-2.00 μg/mL.Their MICs were about 2-4 and 2-8 fold stronger than that of its o-riginal form and levofloxacin hydrochloride respectively;the MBCs were 0.25 μg/mL-4.00 μg/mL and about 2-8 fold stronger than that of its original form and levofloxacin hydrochloride.Invivo ,the tosufloxacin nanoemulsion in low(40 mg/L)and middle doses(60 mg/L)significantly reduced the mortality and weight loss of the model chicks caused by Salmonella typhimurium,obviously ameliorated the symptoms.It can be concluded that tosufloxacin nanoemulsion as a novel type of drug can enhance the efficacy of tosufloxacin on common bactrerials and expand the clinical application value.关键词
托氟沙星/纳米乳/抗菌作用Key words
tosufloxacin/nanoemulsion/antibacterial activity分类
农业科技引用本文复制引用
高娴,欧阳五庆,杨鸣琦,王波臻,郑寅,李梦云,刘岳..托氟沙星纳米乳的体内外抗菌作用研究[J].动物医学进展,2015,(11):44-48,5.基金项目
农业科技成果转化资金项目 （）
