动物营养学报Issue(10)：3256-3262,7.DOI:10.3969/j.issn.1006-267x.2015.10.034
Illumina MiSeq测序平台测定蒙古羊瘤胃液相和固相菌群多样性
Bacteria Community Diversity of Liquid and Solid Phases of Ruminal Contents of Mongolian Sheep Analyzed by Illumina MiSeq Platform
摘要
Abstract
This experiment was conducted to compare bacteria community diversity of liquid and solid phases of ruminal contents of Mongolian sheep. The samples of liquid and solid phases of ruminal contents from 5 Mongolian sheep were investigated using 16S rRNA amplicon high-throughput sequencing technique by Illumi-na MiSeq platform, and the results were analyzed by UPARSE-operational taxonomic units ( OTU) and un-weighted pair group method with arithmetic mean ( UPGMA) . The results showed that a total of 91 971 se-quences and 1 871 OTUs across all samples were observed, and the average number of sequence and OTU was 13 138 and 267 per sample, respectively. Compared between the samples of liquid and solid phases: at phyla level, dominant bacteria with significant differences were Bacteroidetes and Firmicutes, and the percentage of Bacteroidetes of the samples of liquid and solid phases was 67% and 45%, respectively;at genus level, domi-nant bacteria with significant differences were Prevotella and an unknown genus, dominant bacterium of liquid phase was Prevotella whose percentage reached 53%, and that of solid phase was the unknown genus whose percentage reached 32%; at species level, dominant bacteria with significant differences were Prevotella ru-minicola, and the percentage of liquid and solid phases reached 41% and 11%, respectively. The alpha diversi-ty of solid phase was higher compared with that of liquid phase, and the samples from the same animal clus-tered together by principal component analysis ( PCA) and principal coordinate analysis ( PCoA) . In conclu-sion, both the contents of liquid and solid phases have high diversity of bacteria, and the diversity of solid phase is higher compared with that of liquid phase.关键词
Illumina MiSeq/液相/固相/细菌/多样性Key words
Illumina MiSeq/liquid phase/solid phase/bacteria/diversity分类
农业科技引用本文复制引用
曾燕,简平,倪学勤,周毅,祝辉,曾东..Illumina MiSeq测序平台测定蒙古羊瘤胃液相和固相菌群多样性[J].动物营养学报,2015,(10):3256-3262,7.基金项目
教育部留学回国人员科研启动基金资助项目(200835-1) （200835-1）
四川省学术带头人培养基金资助项目 （）
