动物营养学报Issue(10)：3270-3278,9.DOI:10.3969/j.issn.1006-267x.2015.10.036
浒苔添加比例与微生物发酵对幼刺参生长、消化和非特异性免疫的影响
Effects of Enteromorpha prolifera Adding Ratio and Microbial Fermentation on Growth, Digestion and Non-Specific Immune of Juvenile Sea Cucumbers (Apostichopus japonicus Selenka)
摘要
Abstract
A 46-day feeding trial was conducted to evaluate the effects of adding Enteromorpha prolifera and microbial fermentation on growth, digestion and non-specific immune of juvenile sea cucumbers ( Apostichopus japonicus Selenka) . Main sources included Enteromorpha prolifera, Sargassum polycystum, Sargassum thun-bergii, Ulva lactuca and so on. Five diets were formulated to contain 0, 10%, 20%, 30% and 40% of Entero-morpha prolifera, respectively. Half of each diet was fermented by the suspension of Brewer’ s yeast. The trial finally had 10 experimental diets. Each diet was randomly fed to one group with 3 replicates of 30 sea cucum-ber [average body weight of (3.94±0.46) g]. The results showed as follows: 1) the weight gain rate ( WGR) , specific growth rate ( SGR) and feed efficiency ( FE) of sea cucumbers were increased with Entero-morpha prolifera adding ratio increasing among the non-fermented groups, and the group of 40% Enteromor-pha prolifera was the best;the WGR, SGR and FE of sea cucumbers were firstly increased and then decreased with Enteromorpha prolifera adding ratio increasing among the fermented groups, and the group of 30% Enter-omorpha prolifera was the best. The WGR, SGR and FE in the group of 30% and 40% Enteromorpha prolif-era were not significantly different ( P>0.05) , but all of them were significantly higher than those in the group of 10% Enteromorpha prolifera ( P<0.05) . The growth performance indices of sea cucumbers were not signifi-cantly affected by microbial fermentation (P>0.05). 2) The intestinal cellulase activity was increased in the first stage and then decreased with Enteromorpha prolifera adding ratio increasing among the non-fermented groups. However, the intestinal cellulase activity tended to increase with Enteromorpha prolifera adding ratio increasing among the fermented groups. In the same adding ratio, the intestinal cellulase activity in the fermen-ted groups was higher than that in the non-fermented groups, and the significant differences were found when adding ratios were 10%, 20% and 40% (P<0.05). There was significant interaction between yes or no fer-mentation and Enteromorpha prolifera adding ratio on the intestinal cellulase activity ( P<0.05) , but no signifi-cant interactions on intestinal amylase and trypsin activities ( P>0.05) . 3) The superoxide dismutase ( SOD) , acid phosphatase ( ACP) and alkaline phosphatase ( AKP) of coelomic fluid were not significantly affected by yes or no fermentation and Enteromorpha prolifera adding ratio ( P>0.05) , and the yes or no fermentation and Enteromorpha prolifera adding ratio had no significant interactions on them. It can be concluded that the opti-mal adding ratio of Enteromorpha prolifera in the diet of juvenile sea cucumber is between 30% and 40%. And microbial fermentation can promote the intestinal cellulase activity of juvenile sea cucumber to some extent.关键词
浒苔/刺参/发酵/生长/纤维素酶/非特异性免疫Key words
Enteromorpha prolifera/sea cucumbers ( Apostichopus japonicus Selenka )/fermentation/growth/cellulase/non-specific immune分类
农业科技引用本文复制引用
李猛,廖梅杰,王印庚,常青,李彬,张振,范瑞用..浒苔添加比例与微生物发酵对幼刺参生长、消化和非特异性免疫的影响[J].动物营养学报,2015,(10):3270-3278,9.基金项目
青岛市战略性新兴产业培育计划项目(13-4-1-65-hy) （13-4-1-65-hy）
青岛市民生科技计划项目“浒苔生物饲料生产及利用技术”(14-9-3-3-hy) （14-9-3-3-hy）
青岛市市南区科技发展资金项目(2014-14-009-SW) （2014-14-009-SW）
