动物营养学报2015，Vol.27Issue(11)：3549-3557,9.DOI:10.3969/j.issn.1006-267x.2015.11.028
不同牧草来源饲粮对奶牛瘤胃液中细菌群落结构多样性的影响
Diversity of Bacterial Communities in Rumen Fluid of Dairy Cows Affected by Forage Sources in Diet
摘要
Abstract
This study was conducted to investigate the effects of forage sources in diet on the diversity of bacte-rial communities in rumen fluid of dairy cows. Eight Holstein dairy cows [ ( 632 ± 12 ) kg of body weight;(135±16) days in milk] were used in a replicated 4×4 Latin square design, and each group contained two cows. Oat hay, Leymus chinensis, rice straw and alfalfa were used as forage sources to form four isonitroge-nous and isocaloric diets, which were based on corn silage and had equivalent neutral detergernt fiber ( NDF) and non-fiber carbohydrates ( NFC) contents; each test period lasted for 21 days with the first 14 days for a-daption. The results showed as follows: 1 ) Bacteroidertes, Firmicutes, Spirochaetes and Fibrobacteres were the most abundant in rumen under phyla level; Prevotella, Butyrivibrio, Fibrobacter and Treponema were the most abundant under genus level. 2) Similarity of the bacterial community feeding the same diet was high un-der genus level, and the genetic distance was near in rumen fluid of cows fed oat hay, rice straw and alfalfa. 3) The four groups comprised of 9 329 operational taxonomic units ( OTUs) at the 97% similarity level and 4 120 OTUs were shared, which accounted for 44.16% of the total OTUs. The results indicate that cows fed di-ets with different forage sources with equivalent NDF and NFC contents have similar diversity of bacterial com-munities.关键词
细菌群落结构/高通量测序/牧草来源/瘤胃Key words
bacterial community/high throughput sequencing/forage sources/rumen分类
农业科技引用本文复制引用
徐俊,胡丽芳,侯玉洁,孙建勇,赵国琦..不同牧草来源饲粮对奶牛瘤胃液中细菌群落结构多样性的影响[J].动物营养学报,2015,27(11):3549-3557,9.基金项目
国家自然科学基金(31560647) （31560647）
十二五国家科技支撑计划(2011BAD17B03) （2011BAD17B03）
