动物营养学报2015，Vol.27Issue(11)：3620-3627,8.DOI:10.3969/j.issn.1006-267x.2015.11.036
紫色达利菊缩合单宁对大肠杆菌的抑制作用
Inhibiting Effects of Condensed Tannins from Purple Prairie Clover on Escherichia coli
摘要
Abstract
This experiment was conducted to evaluate inhibiting effects of purple prairie clover ( PPC, Dalea purpurea Vent.) on Escherichia coli (E. coli) growth prevalence in the rumen and large intestine and its con-densed tannins ( CT ) antibacterial mechanism. Ruminal and large intestinal environments were simulated in vitro, non-pathogenic E. coli ATCC 25922 and pathogenic E. coli O157:H7 were used as test strains, and the strains were cultured with PPC and alfalfa ( no CT, control) as substrates, respectively. Bacteria number was determined after cultured for 0, 1.5, 3.0, 6.0 and 24.0 h, respectively. With the E. coli ATCC 25922 as test model, the bacteria suspensions supplemented with CT from PPC and sainfoin were mixed with o-nitrophenyl-β-D-galactoside (ONPG) solution, meanwhile, the mixture of bacteria suspension without CT and ddH2O was used as control. O-nitrophenyl amount, the hydrolysis product of β-D-galactosidase, was determined after cul-tured for 0, 40, 80, 120 and 160 min, respectively, to analysis the change of cell inner-membrane permeabili-ty. The effects of different pH and temperatures on CT actions were also observed. The results revealed as fol-lows:1) compared with alfalfa, PPC had significant inhibiting effect on growth of E. coli from rumen (6.0 and 24.0 h) and large intestine (1.5 to 24.0 h) (P<0.05). 2) CT significantly increased o-nitrophenyl a-mount ( P<0.05); differently from actions of CT from sainfoin, actions of CT from PPC were affected by pH and temperature. The results indicate that PPC showed inhibiting effect on E. coli growth in bovine rumen and large intestine in vitro incubation;its CT can increase inner membrane permeability of E. coli, however, action of inhibition associated with temperature and pH.关键词
紫色达利菊/缩合单宁/大肠杆菌/抑制Key words
PPC/condensed tannins/E. coli/inhibition分类
农业科技引用本文复制引用
刘秀丽,王玉玺,李元恒,许中军,金龙,侯勇跃..紫色达利菊缩合单宁对大肠杆菌的抑制作用[J].动物营养学报,2015,27(11):3620-3627,8.基金项目
国家自然科学基金项目(31460652) （31460652）
公益性行业(农业)科研专项课题(201303040-11) （农业）
