福建农业学报Issue(8)：727-730,4.
H5、H9亚型禽流感病毒及鸭坦布苏病毒多重 PCR 检测方法的建立及初步应用
Establishment and Application of Multiplex PCR Assay for Detection of H5、H9 Subtypes Avian Influenza Virus and Duck Tembusu Virus
摘要
Abstract
The aim of this study is to establish a rapid multiplex PCR assay for detection of H5,H9 subtype avian influenza virus (AIV)and duck Tembusu virus (DTMUV). According to the HA gene sequences of H5 and H9 AIV and NS5 gene of DTMUV in GenBank,three primer sets specific to these three kinds of viruses were designed,respectively. The multiplex PCR for simultaneous detection of AIV (H5,H9 )and DTMUV was established,and was applied to clinical testing for three kinds of viruses. The specificity and sensitivity of the multiplex PCR were tested. The results showed that the assay could specifically amplify the gene fragments of H5 AIV (380 bp),H9 AIV (732 bp),and DTMUV (250 bp),and were all negative for detection of other duck viruses.This method showed a sensitive of 533 pg·μL-1 for H5 AIV,56 pg·μL-1 for H9,and 6.6 ng·μL-1 for DTMUV.Additionally,the detection results of 200 clinical samples indicated that this multiplex PCR method proved a a rapid,sensitive and specific tool for clinical samples detection.关键词
H5 亚型禽流感病毒/H9 亚型禽流感病毒/鸭坦布苏病毒/多重 PCRKey words
H5 AIV/H9 AIV/duck Tembusu virus/multiplex PCR分类
农业科技引用本文复制引用
李海琴,傅光华,黄瑜,韦启鹏,唐维国..H5、H9亚型禽流感病毒及鸭坦布苏病毒多重 PCR 检测方法的建立及初步应用[J].福建农业学报,2015,(8):727-730,4.基金项目
国家水禽产业技术体系建设专项（CARS-43-33）；江西省对外科技合作计划项目 （）
