福建农业学报Issue(8)：784-788,5.
杏鲍菇原生质体再生条件初探
A Preliminary Study on Regeneration of Pleurotus eryngii Protoplast
张鹏 1赵光辉 2林金秀 1朱坚 3庄卫东1
作者信息
- 1. 福建省泉州市农业科学研究所，福建 泉州 362212
- 2. 福建省福州市农业科学研究所，福建 福州 350002
- 3. 福建农林大学园艺学院，福建 福州 350002
- 折叠
摘要
Abstract
Effect of regenerating method,osmotic pressure stabilizer,culture medium,age of mushroom,etc.on the regeneration of Pleurotus eryngii protoplast was investigated.The results showed that the protoplasts from the 5-7 days old mycelia yielded the most desirable results when cultured in a yeast peptone regeneration medium with up to 0.9% of NaCl as the osmotic stabilizer.Lowered temperature,shortened time and reduced enzyme concentration could help minimize enzymatic hydrolysis in the preparation which would benefit the protoplast regeneration.关键词
杏鲍菇/原生质体/再生率Key words
Pleurotus eryngii/protoplast/regeneration rate分类
农业科技引用本文复制引用
张鹏,赵光辉,林金秀,朱坚,庄卫东..杏鲍菇原生质体再生条件初探[J].福建农业学报,2015,(8):784-788,5.
