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罗源湾养殖大黄鱼虹彩病毒的PCR检测

杨小强

福建畜牧兽医Issue(2):5-7,8,4.
福建畜牧兽医Issue(2):5-7,8,4.

罗源湾养殖大黄鱼虹彩病毒的PCR检测

PCR detection of cage cultured large yel ow croaker Iridovirusin in the Luoyuan bay, Fujian province

杨小强1

作者信息

  • 1. 福州市海洋与渔业技术中心 福州 350026
  • 折叠

摘要

Abstract

A white gill disease, caused by Iridovirus perhaps, broke out in cage cultured large yellow croaker (Pseudosciaena crocea) in the Luoyuan Bay from July to October in 2012. The genome DNA of disease fish was extracted from gill, liver, spleen, kidney and go-nads respectively, and used for the PCR detection of Iridovirus. As a result, the virus gene was detected in the genome DNA from liv-er, spleen and kidney. Through analysis, the sequences ly1 and ly2 acquired were of high identity with the reported sequences of large yellow croaker(P.crocea) Iridovirus AY779031 and red sea bream(Pagrosomus major) Iridovirus AB104413. During July to October the detection rate of sick fish infected by Iridovirus was 10%to 65%, and it did follow that the Iridovirus had a direct bearing on the fre-quent death of large yellow croaker (P.crocea) with white gill disease in the Luoyuan Bay.

关键词

罗源湾/大黄鱼/虹彩病毒/PCR

Key words

Luoyuan Bay/Large Yellow Croaker(Pseudosciaena crocea)/Iridovirus/PCR

引用本文复制引用

杨小强..罗源湾养殖大黄鱼虹彩病毒的PCR检测[J].福建畜牧兽医,2013,(2):5-7,8,4.

福建畜牧兽医

1003-4331

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