福建畜牧兽医Issue(3)：11-14,4.
人血清白蛋白基因在鹌鹑两种细胞上表达的研究
A study about the expression of human serum albumin genes in two kinds of quail cell
摘要
Abstract
we constructed the primary oviduct epithelium and quail embryo fibroblasts cell (QEF)of quail were cultured as testing platform. Post-recombinant expression vector of human serum albumin transfected into the two cells using liposome. The expression of report gene EGFP mainly in cytoplasm was observed under fluoroscope. The results showed thatthere was no expression at fibroblasts of the recombination vectors, but it was expressed in oviduct epithelium primary.Genomic DNA of transfected cells were extracted, specific primers were detected,reporter gene-positive cell clones were detect by using PCR method,and molecular weight of Quail oviduct epithelial cell secretory protein were fixed by using SDS-PAGE.The results showed that there were HAS which molecular weight is 68 kDa in transfected quail oviduct epithelial cellculture supernatant,which proved that the constructed expression vector was effective.And the expression of the initial components have been integrated into the quail oviduct epithelial cells positive for the chromosome.关键词
人血清白蛋白/鹌鹑/细胞表达Key words
human serum albumin/quail/cell expression引用本文复制引用
洪志勇,李玉芳,李明,张兴峰,林秀娇,李鸿翔,庄益芬,张文昌..人血清白蛋白基因在鹌鹑两种细胞上表达的研究[J].福建畜牧兽医,2013,(3):11-14,4.基金项目
福建省自然科学基金项目（2007J0042）资助。 （）
