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猪蛔虫各期虫体基因片段(2G04)表达情况分析OA

The analysis of the expression condition of gene(2G04)in polypide in different developmental phases of Ascaris sum

中文摘要英文摘要

采用半定量RT-PCR(Reverse Transcription-Polymerase Chain Reaction)方法,探讨编码铁蛋白基因(命名为2G04)在猪蛔虫不同发育期虫体的表达丰度。结果表明该基因在雄虫中没有检测到表达量,在其他各期幼虫均有较高表达,其中在虫卵中表达量最高。试验结果可为猪蛔虫的发育生物学和免疫防治奠定基础和提供科学依据。

This experiment adopted semiquantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction)method to discuss the expression abundance of coding ferritin gene (named 2G04) in polypide in different developmental phases .The result showed that no expression was found in maleworm for this gene, and there have high expression in other different stages,among which the highest expression was found in eggs.The experimental result lays the foundation and provid…查看全部>>

曾玉梅

福建省武平县下坝乡畜牧兽医站 364309

猪蛔虫各期虫体半定量RT-PCR基因表达

Ascaris sumdifferent stages of Ascaris suumsemi-quantitative RT-PCRdifferentially expressed gene

《福建畜牧兽医》 2015 (5)

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