分析化学Issue(11)：1620-1628,9.DOI:10.11895/j.issn.0253-3820.150565
核酸外切酶辅助的信号放大策略在生化分析中的应用进展
Application Progress of Exonuclease-assisted Signal Amplification Strategies in Biochemical Analysis
摘要
Abstract
As an important member of tool enzymes, exonuclease is a kind of hydrolytic enzymes without strict base sequence dependent. In recent years, by taking advantage of different hydrolysis ways of exonuclease and nanotechnology, cycle effect of enzyme digestion, aptamer, non Watson-Crick base pairing system by metal ions, fluorescent nucleic acid probes, electrochemical methods etc. , a series of exonuclease-assisted signal amplification strategies have been developed, which played a very key role in improving the sensitivity of detection methods. Therefore, exonuclease has been widely used in high sensitive detection of nucleic acids, proteins, ions, small molecules and so on. To understand it better and apply it well in the future, the application progress of exonuclease-assisted signal amplification strategies in biochemical analysis has been summarized in this review.关键词
分析检测/工具酶/信号放大/核酸外切酶/评述Key words
Analysis and detection/Tool enzymes/Signal amplification/Exonuclease/Review引用本文复制引用
文立,徐凤州,何晓晓,王柯敏,何定庚,卿太平,邹振..核酸外切酶辅助的信号放大策略在生化分析中的应用进展[J].分析化学,2015,(11):1620-1628,9.基金项目
本文系国家自然科学基金项目(Nos.21322509,21305035,21305038,21190044)资助 （Nos.21322509,21305035,21305038,21190044）
