高等学校化学学报Issue(11)：2349-2354,6.DOI:10.7503/cjcu20150688
非表面活性剂模板溶胶-凝胶法制备磁性介孔二氧化硅实现纤维二糖酶的原位固定
In Situ Encapsulation of Cellubiase Enzyme in Magnetic Mesoporous Silica via the Nonsurfactant-templated Sol-gel Method
摘要
Abstract
Nonsurfactant-templated sol-gel method is an advanced technology for enzyme encapsulation. The immobilized enzyme can be reused many times, so that can reduce the cost. It has a broad application prospect in biological catalysis. D-fructose is used as non-surfactant template to generate mesopores. The average diameter of the mesoporous silica is 3. 2 nm. And they significantly improve the activity of the immobilized enzyme. The best result is 70% of free enzyme. On the other hand, the mesopores are smaller than the size of cellobiase enzyme. So the enzyme will not leach out. That can help avoiding enzyme leaching. The magnetic nanoparticles can make the separation and recycle of immobilized enzyme easier and more feasi-ble. The incorporation of magnetic nanoparticles has little effect on the enzyme activity. The magnetic encap-sulated enzyme can be easily recycled by magnet force. More than 83% remained after 10 cycles. The process is simple and biocompatible as the sol-gel reaction occurs inaqueous phase and neutral pH.关键词
酶固定化/纤维二糖酶/介孔二氧化硅/磁性纳米颗粒/溶胶-凝胶反应/非表面活性剂Key words
Enzyme encapsulation/Cellobiase enzyme/Mesoporous silica/Magnetic nanoparticles/Sol-gel reaction/Non surfactant template分类
化学化工引用本文复制引用
张翔,李书润,翟文韬,曹莹泽,齐宏旭,吉岩,危岩..非表面活性剂模板溶胶-凝胶法制备磁性介孔二氧化硅实现纤维二糖酶的原位固定[J].高等学校化学学报,2015,(11):2349-2354,6.基金项目
国家“九七三”计划项目(批准号:2011CB935701)资助.覮 Supported by the National Basic Research Program of China(No.2011CB935701) （批准号:2011CB935701）
