干旱地区农业研究Issue(4)：104-107,113,5.
盐胁迫下己唑醇铜配合物对小麦幼苗脯氨酸合成的影响
The effect of hexaconazole -Cu complex on proline accumulation in wheat seedlings under NaCl stress
摘要
Abstract
The hexaconazole-Cu complex ,synthesized by hexaconazole and copper acetate ,was used to soak the seed of wheat to ameliorate the wheat seedlings salinity stress induced by sodium chloride (NaCl) .The effect of hexa-conazole-Cu complex on the anti-salinity stress of wheat seedlings was evaluated by proline accumulation level and the gene expression level of Δ1-pyrroline-5-carboxylate synthetase (P5CS) and Δ1-pyrroline-5-carboxylate reduc-tase (PC5R) of wheat seedlings .The results showed that in the presence of 200 mmol・L-1 NaCl ,the proline content of the 0 .03 mmol・L-1 complex treated wheat seedlings was 55 .44 μg・g-1 ,which was 13 .68% higher than that in hexa-conazole treatment ,and 58 .45% higher than that in copper acetate treatment .The gene expression levels of P5CS and P5CR were quantitated by semiquantitative RT-PCR analysis .The results showed that the hexaconazole -Cu complex enhanced the transcript levels of P5CR and P5CS .After 6 h treatment of the complex in the presence of 100 mM NaCl , the P5CS express level reached the maximum ,and was significantly higher than in hexaconazole treatment .It was obvi-ous that the hexaconazole -Cu complex enhanced the proline contents ,thereby played a protective role in the salinity stress of wheat seedlings .关键词
己唑醇铜配合物/脯氨酸/半定量PCR/盐胁迫Key words
hexaconazole-Cu complex/proline/semiquantitative RT -PCR/salt stress分类
农业科技引用本文复制引用
毕彦博,李婕,赵佳,张晓庆,潘红艳,步怀宇..盐胁迫下己唑醇铜配合物对小麦幼苗脯氨酸合成的影响[J].干旱地区农业研究,2013,(4):104-107,113,5.基金项目
国家自然科学基金项目（21073142，31201534）；陕西省教育厅基金 （）
