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外源 NO 对干旱胁迫下玉米幼苗膜脂过氧化的调节效应

王芳 赵有军 王汉宁

干旱地区农业研究Issue(5):75-79,5.
干旱地区农业研究Issue(5):75-79,5.DOI:10.7606/j.issn.1000-7601.2015.05.14

外源 NO 对干旱胁迫下玉米幼苗膜脂过氧化的调节效应

Regulation of membrane lipid preoxidation by nitric oxide in maize seedlings under drought stress

王芳 1赵有军 1王汉宁1

作者信息

  • 1. 甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室/甘肃农业大学农学院,甘肃 兰州 730070
  • 折叠

摘要

Abstract

In order to explore the influences of oxidative damage by nitric oxide (NO ) on maize seedlings under drought stress ,a pot experiment to investigate the effects of exogenous nitric oxide donors (sodium nitroprusside ,SNP) at concentrations of 0 .01 ,0 .05 ,0 .1 ,0 .5 mmol·L-1 and 1 .0 mmol·L-1 on membrane lipid peroxidation was carried out using Zhengdan 958 as experimental material .The results showed that appropriate concentration (0 .05 mmol·L-1 ) of exogenous NO could relieve the damage from drought stress , significantly improving the activity of protective enzymes SOD and CAT .Compared with those under drought stress ,the SOD and CAT activities were increased by 23 .89% and 87 .78% ,respectively . Proline , soluble sugar , soluble protein and chlorophyll content were increased by 23 .89% , 87 .78% ,43 .44% ,39 .84% ,41 .33% and 21 .45% ,respectively .However ,MDA content and membrane permeabil-ity were significantly reduced by 11 .76% and 51 .17% ,respectively .There were dosage effects of exogenous nitric oxide donor (SNP) on alleviation of drought stress in maize seedlings ,which significantly enhanced the drought tolerance of maize seedlings .

关键词

一氧化氮/干旱胁迫/玉米幼苗/膜脂过氧化

Key words

nitric oxide/drought stress/maize seedling/membrane lipid peroxidation

分类

农业科技

引用本文复制引用

王芳,赵有军,王汉宁..外源 NO 对干旱胁迫下玉米幼苗膜脂过氧化的调节效应[J].干旱地区农业研究,2015,(5):75-79,5.

基金项目

973计划前期研究专项(2012CB722902);甘肃农业大学盛彤笙科技创新基金 ()

干旱地区农业研究

OACSCDCSTPCD

1000-7601

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