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马铃薯转 GhABF2转录因子苗耐盐性研究

裴怀弟 李忠旺 张艳萍 陈玉梁

干旱地区农业研究Issue(5):90-95,6.
干旱地区农业研究Issue(5):90-95,6.DOI:10.7606/j.issn.1000-7601.2015.05.17

马铃薯转 GhABF2转录因子苗耐盐性研究

Characteristics of salt tolerance capability of potato seedlings overexpressing GhABF2

裴怀弟 1李忠旺 1张艳萍 1陈玉梁1

作者信息

  • 1. 甘肃省农业科学院生物技术研究所,甘肃 兰州 730070
  • 折叠

摘要

Abstract

In order to improve adaptability of crops to adversity ,transgenic potato seedlings with GhABF2 gene were employed as study materials to investigate their drought tolerance capability .Results showed that under the conditions of NaCl stress ,two lines with GhABF2 transgene displayed similar variations to the control plants in terms of dry weight , fresh weight ,physiological and biochemical indexes .Based on the phenotypic variations ,it was found that the transgenic plants were significantly strong in drought resistance .With the increase of NaCl concentrations ,compared with non-trans-genic plants ,biomass of two transgenic plants appeared to be significantly increased .In addition ,with the increase of NaCl concentrations ,chlorophyll content of all seedlings investigated became decreased ,whereas soluble sugar ,MDA , proline ,SOD activity ,and POD activity all appeared to be increased .Soluble protein content did not display significant variations .On basis of the plant growth status and its physiological and biochemical indexes , two transgenic plants showed stronger resistance than non-transgenic plants .

关键词

马铃薯/GhABF2/转基因/苗期/盐胁迫

Key words

potato/GhABF2/transgene/potato seedling/salt-stress

分类

农业科技

引用本文复制引用

裴怀弟,李忠旺,张艳萍,陈玉梁..马铃薯转 GhABF2转录因子苗耐盐性研究[J].干旱地区农业研究,2015,(5):90-95,6.

基金项目

甘肃省农业生物技术研究与应用开发项目(GNSW-2010);甘肃省农业科学院农业科技创新专项(2012GAAS12-1);甘肃省农业科学院中青年基金项目 ()

干旱地区农业研究

OACSCDCSTPCD

1000-7601

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