甘肃农业科技Issue(3):3-5,3.DOI:10.3969/j.issn.1001-1463.2013.03.001
2个新育成黄芪品种(系)的RAPD研究
Study on RAPD of Two New Breeding Astragalus membranaceus Varieties(Strains)
摘要
Abstract
In the present study, RAPD molecular marker technology were used for PCR amplification reaction of the two new breeding Astragalus membranaceus varieties (strains) Longqi 1 and HQN03-03, to identify two Astragalus membranaceus varieties (strains) of between genetic diversity and genetic relationship . The results showed that 12 selected primers from Longqi 1 were amplification out 112 sites which can amplification out 9.3 sites of the average each primer, 13 selected primers from HQN03-03 were amplification out 107 sites which, can amplification out 8.2 site of the average each primer. The Lord band of two Astragalus membranaceus were basically identical that the genetic background of between two varieties (strains) had great similarity, but sub-band have difference of different degree, the two varieties (strains) has certain genetic differences, also it embodies the Astragalus varieties(strains) resources were the more abundant genetic diversity in Gansu province.关键词
黄芪/RAPD/新品种(系)/鉴别Key words
Astragalus membranaceus/RAPD/New varieties (strains)/Identification分类
农业科技引用本文复制引用
刘效瑞,杨宁,王春明,尚虎山,汪淑霞,王兴政..2个新育成黄芪品种(系)的RAPD研究[J].甘肃农业科技,2013,(3):3-5,3.基金项目
甘肃省农业科学院农业科技创新专项(2010GAAS13)部分研究内容 (2010GAAS13)