医学分子生物学杂志Issue(5)：258-262,5.DOI:10.3870/j.issna.1672-8009.2015.05.003
单个液滴内胚胎培养数量及同培养非移植胚胎质量对妊娠结局的影响
Effect of the Quality of Co-Cultured Non-Transfer Embryo and Em-bryo Number in A Single Droplet on Pregnancy Outcome
摘要
Abstract
Objective To examine the effect of the quality of co-cultured non-transfer embryo in a single droplet on pregnancy outcome, and explore the effect of culture method and co-cultured non-transfer embryos on implantation potential.Methods Clinical data of 1587 IVF-ET patients were retrospectively analyzed.Patients were divided into different groups according to the droplets from which the embryo came.In group A, the transferred embryos were from a single droplet.In group B, at least one embryo was from co-culture system.In group C, the embryo was from two dif-ferent droplets.Group B was divided into two subgroups, group B1 ( top quality embryo) and group B2 ( non-top quality embryo) , according to the co-cultured embryo quality.The clinical pregnancy outcomes were compared between these groups.Results There was no significant difference in basic data between these groups.The number of transferred embryo was lower in group A than in other groups, and that was higher in group B1 than in group C and group B2.The implantation rate was higher in group A than in group B and group B2 , and greater in group B1 and group C than in group B2 ( P<0.05) .The clinical pregnancy rate in group A, group B1 and group C was higher than in group B2.When two embryos were transferred, the implantation rate was significantly en-hanced in group A relative to group B, and the clinical pregnancy rate was higher in group A, group B1 and group C than in group B2.When three embryos were transferred, the implantation rate was significantly increased in group C when compared with group B2 ( P<0.05 ) .Conclusion Patients who were transferred with co-cultured top quality embryo from the same single droplet have better implantation rate and clinical pregnancy rate, and transfer of co-cultured non-top quality em-bryo may have an adverse effect on clinical outcomes.关键词
微滴/共培养/非移植胚胎/临床妊娠率/种植率Key words
droplet/co-culture/non-transfered embryo/the implantation rate/the clinical pregnancy rate分类
医药卫生引用本文复制引用
尹太郎,徐望明,杨菁,丁锦丽,吴庚香,王雅琴,程丹,肖卓妮,罗金,谢青贞,龙文..单个液滴内胚胎培养数量及同培养非移植胚胎质量对妊娠结局的影响[J].医学分子生物学杂志,2015,(5):258-262,5.基金项目
国家自然科学基金（No．81401276）This work was supported by a grant from the National Natural Science Foundation of China （）
