国际口腔医学杂志Issue(6)：655-658,4.DOI:10.7518/gjkq.2015.06.010
牙龈卟啉单胞菌菌毛蛋白的重组及表达
Recombination and expression of fimbriae protein for Porphyromonas gingivalis
摘要
Abstract
Objective This study aimed to clone the type Ⅱ fimA gene of Porphyromonas gingivalis(P. gingivalis) and detect its expression in Escherichia coli(E. coli). Methods The type Ⅱ fimA gene was obtained by polymerase chain reaction(PCR) from the genome of P. gingivalis to construct a prokaryotic expression plasmid pET-FimA. pET-FimA was transformed into E. coli BL21(DE3) competent cells, and the recombination protein was characterized via sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) and Western blot analysis. Results DNA sequencing showed that the fragment was 95% consistent with that of published literature. We successfully constructed prokaryotic expression plasmid pET-FimA. Conclusion The plasmid efficiently expressed the recombinant fimbriae protein of the type ⅡfimA gene of P. gingivalis. A high purity of protein FimA was obtained. FimA has immunogenicity and immune reactivity.关键词
ⅡfimA型牙龈卟啉单胞菌/重组菌毛/蛋白表达Key words
type Ⅱ fimA gene of Porphyromonas gingivalis/recombinant fimbriae protein/protein expression分类
医药卫生引用本文复制引用
肖莉,林玉祥,葛颂..牙龈卟啉单胞菌菌毛蛋白的重组及表达[J].国际口腔医学杂志,2015,(6):655-658,4.基金项目
国家自然科学基金（30760271，81260168）；贵州省优秀青年科技人才培养对象专项资金 （）
