国际病理科学与临床杂志Issue(10)：1788-1793,6.DOI:10.3978/j.issn.2095-6959.2015.10.017
口腔鳞状细胞癌瘤芽与上皮-间质转化相关性分析及临床意义
Correlation analysis between tumor budding and EMT in oral squamous cell carcinoma and its clinical significance
摘要
Abstract
Objective: To investigate the correlation between tumor budding and epithelial-mesenchymal transition (EMT), further explore the relationship with clinical -pathological features and clinical prognosis.Methods: To collect 40 resected specimens from patients with oral squamous cell carcinoma who were admitted in our hospital from 2007 to 2013. hTey were divided into 2 groups according to different number of tumor buddings by HE staining, which were low tumor buddings (≤5) and high tumor buddings (>5). Expression of Vimentin in specimens was explored by immunohistochemistry. hTe relationship between tumor budding and EMT and clinical-pathological features was analyzed. Estimation of overall survival (OS) and progression free survival (PFS) distributions was performed by Kaplan-Meier method and differences between groups were determined by log-rank tests.Results: Low tumor budding group (n=22) and high tumor budding group (n=18) by HE staining revealed no signiifcant difference between intratumoral bud cells and cells in tumor body. No correlation between tumor budding number and ages and gender of patients were investigated. Statistical analysis revealed that tumor budding was associated with tumor size, lymph node metastasis and distant metastasis, clinical stage(P<0.05). In addition, significant association was observed between tumor budding and up-regulation of Vimentin (P=0.001), revealing that tumor budding is associated with EMT of OSCC and morphological feature of EMT. hTe median overall survival time of two groups was 40.4, 26.5 months respectively (χ2=5.265,P=0.012). The median progression free survival time of two groups was 35.8, 22.1 months respectively (χ2=6.744,P=0.009).Conclusion: Tumor budding, which is associated with EMT, represents worse malignant and invasive cancer cells subpopulation. It could be an independent prognostic factor in OSCC.关键词
口腔鳞状细胞癌/上皮-间质转化/瘤芽/波形蛋白/预后分析Key words
oral squamous cell carcinoma (OSCC)/epithelia-mesenchymal transition (EMT)/tumor budding/vimentin/prognostic analysis引用本文复制引用
延丽雅,郭健,陈辉娥,黎松娇..口腔鳞状细胞癌瘤芽与上皮-间质转化相关性分析及临床意义[J].国际病理科学与临床杂志,2015,(10):1788-1793,6.基金项目
东莞市医疗卫生科技计划一般项目(2015105101057)。@@@@hTis work was supported by General Project of Medical and Health Science and Technology Project in Dongguan(2015105101057), P. R. china （2015105101057）
