临床与病理杂志Issue(10)：1810-1815,6.DOI:10.3978/j.issn.2095-6959.2015.10.021
阿司匹林通过上调Ishikawa细胞中HOXA10的表达促进胚胎的黏附
Aspirin promotes embryo implantationin vitro through up-regulation of HOXA10 in Ishikawa cells
孔成才 1雷磊2
作者信息
- 1. 南京大学医学院，南京 210000
- 2. 南京大学医学院附属鼓楼医院生殖医学中心，南京 210008
- 折叠
摘要
Abstract
Objective: To explore the role and mechanism of aspirin in regulating the expression of endometrial receptivity marker gene HOXA10 and embryo implantation.Methods:In this study, Ishikawa cells were treated with different doses of aspirin for indicated time and transfected with siRNAs against HOXA10. hTe expression of HOXA10 mRNA and protein in Ishikawa cells were determined by Real-time PCR and Western blot. Furthermore, the effect of aspirin on embryo implantationin vitro was determined by anin vitro model of BeWo-Ishikawa cell interaction. Results:Compared with the control group treated with DMSO, the expression of HOXA10 mRNA and protein were up-regulated in Ishikawa cells treated with different doses of aspirin. And, the mRNA and protein levels of HOXA10 were highest in Ishikawa cells treated with aspirin at the dose of 10−5 mol/L (P<0.01). Furthermore,aspirin promoted BeWo spheroids attachment to Ishikawa cells, however, the effect of aspirin on BeWo spheroids attachment was inhibited by HOXA10-specific siRNAs in Ishikawa cells.Conclusion: Aspirin may promote embryo implantation through up-regulation of endometrial receptivity marker gene HOXA10.关键词
阿司匹林/子宫内膜容受性/胚胎黏附/HOXA10Key words
aspirin/endometrial receptivity/embryo implantation/HOXA10引用本文复制引用
孔成才,雷磊..阿司匹林通过上调Ishikawa细胞中HOXA10的表达促进胚胎的黏附[J].临床与病理杂志,2015,(10):1810-1815,6.
