神经损伤与功能重建Issue(3)：171-176,6.DOI:10.3870/sjsscj.2013.03.004
维甲酸诱导P19胚胎癌细胞向神经元分化过程中分化相关因子的表达
Expression of Differentiation Related Factors in the Process of Retinoid Acid Inducing P19 Embryonic Carcinoma Cell Differentiation into Neuron Cells
摘要
Abstract
Objective:To explore the expression of differentiation related factors in the process of inducing P19 embryonic carcinoma cells differentiation to neural cells by retinoic acid (RA). Methods:The P19 embryonic carcinoma cells were randomly divided into control group and experimental group. The experimental group was treated by RA while the control group was treated by equal volume anhydrous ethanol without RA. All the cells were induced for 4 days and differentiated for 7-days. The neural differentiation was determined using immuno-cytochemisty method. Real-time qPCR and Western blot were employed to analyse gene and protein changes ofSOX2, NESTIN, TUJ1, Neurod2. Results:At day 7 after differentiation, abundant TUJ1 expression was observed in the experimental group, compared with little expression in the control group. SOX2 was highly expressed in the experimental group while it progressively decreased in the control group. The expression of NESTINincreasedrapidlywhichwassignificantlyhigherinthe experimentalgroupthanthatinthe controlgroup at day 4 after induction but was comparable without significant difference during differentiation. The expression of Neurod2 was observed at day 2 during induction which increased and peaked at d 1 during differentiation. Both Neurod2 and TUJ1 were highly expressed in the experimental group compared with the low expression in the control after RA induction. Conclusion: The RA-induced P19 embryonic carcinoma cell differentiation to neuron-like cellscanbe usedasa vitromodelforneuronaldevelopmentanddifferentiationresearch.关键词
Neurod2/维甲酸/P19胚胎癌细胞/神经元/诱导分化Key words
Neurod2/retinoic acid/P19embryonic carcinoma cell/neuron/inducingdifferentiation分类
医药卫生引用本文复制引用
张文迪,付溪,高金枝,谢雪梅,罗小平..维甲酸诱导P19胚胎癌细胞向神经元分化过程中分化相关因子的表达[J].神经损伤与功能重建,2013,(3):171-176,6.基金项目
国家重点基础研究发展项目（973计划）（No.2005CB5225007）；卫生部临床学科重点项目（No.2010-2012）；“十一五”国家科技支撑计划 （）
