神经损伤与功能重建Issue(4)：239-242,4.DOI:10.3870/sjsscj.2013.04.002
小分子RNA干扰鞘氨醇激酶1表达对APP/PS1小鼠海马细胞凋亡的影响
Effects of Small Interfering RNA Targeting Sphingosine Kinase-1 Gene On Apoptosis of Hip-pocampal Neural Cells in APP/PS1 Mice
摘要
Abstract
Objective:To observe the effects of the recombinant adenovirus vectors that expressed small interfering RNA (siRNA) against the sphingosine kinase1 (sphk1) gene (siRNA-Sphk1) on the apoptosis of hippocampal neural cells in APP/PS1 double transgenic mice. Methods: The recombinant adenovirus vectors were constructed to express siRNA against the sphk1 gene (siRNA-Sphk1). The recombinant adenovirus vectors siRNA-Sphk1 (siRNA-Sphk1 group) or equal volume of saline (saline group) were stereotactic injected into the dentate gyrus of APP/PS1 double transgenic mce. Thirty days later, the ex-pression of green fluorescent protein (GFP), sphk1 protein and caspase-3 protein and the neuronal apopto-sis in the dentate gyrus were examined. Results:The GFP expression was observed in the dentate gyrus of mice in siRNA-Sphk1 group but not in the saline group. Compared with those in the saline group, the sphk1 expression was significantly down-regulated and the caspase-3 expression was significantly up-reg-ulated in siRNA-Sphk1 group ( <0.05). The apoptosis rate in DG in the siRNA-Sphk1 group was signifi-cantly higher than those in the saline and negative control groups ( <0.05). Conclusion:The recombinant adenovirus vectors siRNA-Sphk1 were successfully constructed to silence SPhK1 gene and increase the apoptosis of hippocampus .关键词
鞘氨醇激酶1/基因治疗/阿尔茨海默病/小分子干扰Key words
sphingosine kinase 1/gene therapy/Alzheimer's disease/small interfering分类
医药卫生引用本文复制引用
张远,禹虔,黎刚,孙圣刚,乔娴..小分子RNA干扰鞘氨醇激酶1表达对APP/PS1小鼠海马细胞凋亡的影响[J].神经损伤与功能重建,2013,(4):239-242,4.基金项目
国家自然科学基金(No.81070879) （No.81070879）
