神经损伤与功能重建Issue(6)：471-473,3.DOI:10.3870/sjsscj.2015.06.001
Ghrelin 对小鼠认知功能的影响及机制探讨
Effect and Mechanism of Ghrelin on Cognitive Function in Mice
陈任 1方姝晨 2宁宇 3范里 1陈家禄1
作者信息
- 1. 武汉大学人民医院脊柱外科武汉 430000
- 2. 苏州市吴江区中医医院骨科苏州 215200
- 3. 湖北中医药大学针灸骨伤学院武汉 430065
- 折叠
摘要
Abstract
Objective:To explore the effect and mechanism of Ghrelin on the cognitive function in mice. Methods:Ten wild-type mice (WT group) and 10 CGRP-gene knockout mice (CGRP-/- group)were equally divided as the control subgroup (n=5) and the examination subgroup (n=5). Mice were treated by intraperitoneal injection of 100μL of 0.1 μg/L Ghrelin solution dissolved in PBS (examination subgroup) or with PBS alone (control subgroup). The Morris maze task and probe test were examined in both groups, and then the tissue levels of CGRP, IGF-1 and IGF-1 mRNA in the hippocampus were determined. Results: In the WT group, the escape latency of the examina-tion subgroup was shortened, the time spent in the target area of the examination subgroup was significantly en-hanced, the tissue levels of CGRP, IGF-1 and IGF-1 mRNA were significantly up-regulated (P<0.05) compared to those in the control subgroup. There was no statistically significance between the two subgroups in CGRP-/- group (P>0.05). Compared with the examination subgroup of CGRP-/- group, the escape latency of the examination sub-group of WT group was shorter on days 4 and 5, and the time spent in the target area was longer, and the tissue lev-els of CGRP, IGF-1 and IGF-1 mRNA were significantly higher (P<0.05). Conclusion: Ghrelin may increase the hippocampal expression of IGF-1 via increase of CGRP release from the hippocampus, thus cognitive function was improved.关键词
Ghrelin/胰岛素样生长因子 -1/认知功能Key words
Ghrelin/insulin-like growth factor-1/cognitive function分类
医药卫生引用本文复制引用
陈任,方姝晨,宁宇,范里,陈家禄..Ghrelin 对小鼠认知功能的影响及机制探讨[J].神经损伤与功能重建,2015,(6):471-473,3.
