高校化学工程学报Issue(1)：102-107,6.DOI:10.3969/j.issn.1003-9015.2013.01.017
基于代谢通量分析的SAM和GSH联产溶氧控制策略
The Dissolved Oxygen Control Strategy Based on Metabolic Flux Analysis for Fermentative Co-Production of S-Adenosylmethionine and Glutathione
摘要
Abstract
In order to improve the co-production of SAM and GSH under batch cultivation, the effect of dissolved oxygen (DO) level on fermentative co-production of S-adenosylmethionine (SAM) and glutathione (GSH) in a 5 L stirred fermentor with Candida utilis CCTCC M 209298 was investigated. The metabolic fluxes of intermediates and energy during batch fermentative co-production of SAM and GSH under different agitation rates were calculated by using metabolic flux analysis method. Based on the flux distribution of main intermediates, an optimum DO control strategy of a constant agitation rate of 350 r⋅min−1 maintained during the first 9 h of fermentation followed with a DO level not less than 30% after 9 h was proposed as an efficient approach for high co-production of SAM and GSH. Under this DO control mode, the product of 520.7 mg⋅L−1 for batch co-production of SAM and GSH with C.utilis CCTCC M 209298 was achieved. Furthermore, the reason for the improvement of the co-production of SAM and GSH under the proposed strategy was quantitatively interpreted by the fermentative kinetics, metabolic flux analysis and respiratory quotient, respectively.关键词
S-腺苷甲硫氨酸/谷胱甘肽/联产发酵/代谢通量分析/溶氧控制Key words
S-adenosylmethionine/glutathione/fermentative co-production/metabolic flux analysis/dissolved oxygen control分类
化学化工引用本文复制引用
王大慧,王玉磊,卫功元,邵娜,周捷..基于代谢通量分析的SAM和GSH联产溶氧控制策略[J].高校化学工程学报,2013,(1):102-107,6.基金项目
国家自然科学基金(20906065) （20906065）
