高校化学工程学报Issue(1)：113-118,6.DOI:10.3969/j.issn.1003-9015.2013.01.019
产纤维素酶增效蛋白菌株的筛选及培养条件优化
Screening of the Cellulase-Enhancing-Protein Producing Strain and Optimization of its Culture Conditions
摘要
Abstract
A self-designed screening model was built for the screening of the high-efficient cellulase-enhancing-protein producing strains. A strain (named POEP1), having the ability of producing cellulase-activity-enhancing-protein, was isolated from the soil, and then according to the 16S rDNA gene sequencing analysis, the strain was identified to be Pseudomonas oryzihabitans. Some fermentation conditions were optimized to increase the protein productivity. The most suitable carbon and nitrogen sources were determined to be wheat bran and soybeans meal in the single-factor experiments, respectively. Three key factors such as mass concentrations of wheat bran, soybeans meal and KH2PO4 were selected from eleven factors affecting the protein productivity by Plaekett-Burman design and were further optimized by Box-Behnken design and response surface methodology. The optimal concentrations of these three important factors were obtained as follows 32.3 g⋅L−1 of wheat bran, 24.7 g⋅L−1 of soybeans meal and 4.36 g⋅L−1of KH2PO4. Under the optimal conditions,the cellulase-enhancing activity of 43.31 U⋅mL−1 was obtained, which is 1.7 times higher than that before optimization (15.79 U⋅mL−1) .关键词
纤维素酶增效蛋白/Plackett-Burman 设计/响应曲面/发酵优化Key words
cellulase-enhancing-protein/Plackett-Burman design/response surface methodology/fermentation optimization分类
化学化工引用本文复制引用
覃益民,陶恒,刘幽燕,李青云..产纤维素酶增效蛋白菌株的筛选及培养条件优化[J].高校化学工程学报,2013,(1):113-118,6.基金项目
国家自然科学基金(21066001) （21066001）
广西科学基金资助项目(桂科自0991031) （桂科自0991031）
