高校化学工程学报Issue(3)：393-398,6.DOI:10.3969/j.issn.1003-9015.2013.03.005
野菊花总黄酮的提取及萃取精制研究
Process for Leaching and Extracting Total Flavonoids from Buds of the Chrysanthemum indicum L
摘要
Abstract
In this study, ethanol-water solution was used to leach flavonoids from the flower buds of Chrysanthemum indicum L, and the crude leaching-out products were purified by liquid-liquid extraction. In the leaching process, the influences of ethanol concentration, ratio of solid to liquid, temperature, leaching time and leaching times on yield of the total flavonoids were investigated; while in the liquid-liquid extraction process, a suitable extractant was chosen and the influences of feed concentration, phase ratio and extraction times on the yield of the total flavonoids and purity were studied. It turns out that the suitable conditions are as follows: 1) leaching process:ethanol-water (7:3, v/v) as leaching solution, temperature of 60℃, ratio of solid to liquid of 20:1 mL⋅g−1, leaching time of 1 hour, leaching times of one;2) extraction process:temperature of 25℃, extractant of water-saturated n-butanol, feed concentration of 15 g⋅L−1, phase ratio of 1 and extraction times of three. Under the above conditions, the purity of total flavonoids in the extract after liquid-liquid extraction is 32.4%. Furthermore, it was found that the purities of extracts obtained at the second and third times of extraction are higher than 60%, thus these extracts can be used as raw material for the further separation and purification of flavonoid monomer.关键词
野菊花/总黄酮/提取/萃取Key words
Chrysanthemum indicum L/total flavonoids/leaching/extraction分类
化学化工引用本文复制引用
陈菡,罗能镇,沈剑,相咸高,何潮洪,徐义明,陈新志..野菊花总黄酮的提取及萃取精制研究[J].高校化学工程学报,2013,(3):393-398,6.基金项目
浙江省重点科技创新团队(2009R5000，2010R50017)。 （2009R5000，2010R50017）
