高校化学工程学报Issue(3)：469-475,7.DOI:10.3969/j.issn.1003-9015.2013.03.017
PDMS膜生物反应器封闭循环连续发酵生产ABE
ABE Fermentation in a Continuous and Closed-Circulating Fermentation System with PDMS Membrane Bioreactor
摘要
Abstract
Acetone-Butanol-Ethanol (ABE) fermentation in a PDMS membrane bioreactor with fermentation bacterium of clostridium acetobutylicum was studied, and thereby a continuous and closed-circulating fermentation system for ABE production was established. Two cycles of experiments with the operation mode of intermittent coupling and continuous coupling were conducted respectively, and the two cycles each lasts for 274 h and 300 h separately. Taking the second cycle of experiment as an example, the dry cell weight of 1.68 g⋅L−1, butanol output per unit broth volume of 61.43 g⋅L−1, glucose consumption rate of 1.12 g⋅L−1⋅h−1, butanol volumetric productivity of 0.205 g⋅L−1⋅h−1, butanol specific productivity of 0.122 h−1 and butanol yield of 0.183g⋅g−1 can be obtained. Besides, both glucose consumption rate and butanol productivity of the second cycle are almost twice of those in the first cycle. At least two peak values were observed in the profiles of cell growth curve, glucose consumption curve and butanol production curve, which mean that the microorganism can be revived in the PDMS membrane bioreactor system. The results demonstrate that ABE fermentation (especially for butanol production) in a continuous and closed-circulating fermentation system with PDMS membrane bioreactor is feasible and promising.关键词
ABE发酵/PDMS膜生物反应器/丁醇/渗透汽化Key words
ABE fermentation/PDMS membrane bioreactor/butanol/pervaporation分类
化学化工引用本文复制引用
陈春燕,张俊青,崔海娣,刘光培,李伟佳,应超,肖泽仪..PDMS膜生物反应器封闭循环连续发酵生产ABE[J].高校化学工程学报,2013,(3):469-475,7.基金项目
国家自然科学基金(20776088)。 （20776088）
