广西科学Issue(4)：445-448,453,5.
双肾藤茎枝甾体类化学成分的分离鉴定
Separation and Identification of Steroids from the Stem of Bauhinia glauca subsp.hupehana
摘要
Abstract
Objective]The chemical constituents from the stem of medicinal plant Bauhinia glauca subsp.hupehana were systematically investigated in order to determine its medicinal materials.[Methods]The compounds were isolated and purified by using a combination of vari-ous chromatographic techniques including column chromatography over silica gel,polyamide and Sephadex LH-20 gel,and their structures were identified by physicochemical and spectral analyses.[Results]Detailed chemical constituents investigation of the stem of B .glauca subsp. hupehana led to the isolation of six steroids compounds by various chromatographic methods, whose structures were fully determined as(24R)-5α-stigmastane-3,6-dione (1),(22E ,24R)-ergosta-4,6,8(14),22-tetraen-3-one (2),(24R)-5α-stigmast-4-en-3,6-dione (3),stigmast-4-en-3-one (4)andβ-sitosterol (5),β-daucosterol (6).[Conclusion]The steroids isolated from the stem of B . glauca subsp. hupehana comprise sterols, sterones and steroid glycosides,of which the first two were obtained from genus Bauhinia for the first time.关键词
双肾藤/甾体类化合物/分离纯化/结构鉴定Key words
Bauhinia glauca subsp.hupehana/steroids/isolation and purification/structural identification分类
医药卫生引用本文复制引用
苏志维,陈波,何碧娟,高程海,王一兵,马仲辉..双肾藤茎枝甾体类化学成分的分离鉴定[J].广西科学,2015,(4):445-448,453,5.基金项目
广西科学院基本科研业务费项目(15YJ22 HY06)和广西自然科学基金项目(2012GXNSFEA053001)资助。 （15YJ22 HY06）
