广西医学Issue(4)：418-421,4.DOI:10.11675/j.issn.0253-4304.2013.04.10
大鼠肺动脉高压动物模型的建立及磷酸二酯酶1C的表达
Establishment of A Pulmonary Arterial Hypertension Model in Rats and Expression of Phosphodiesterase 1C
郑晓宇 1覃家锦 2李波 2何巍 2冯旭2
作者信息
- 1. 广西医科大学附属南宁市第一人民医院心胸外科，南宁市 530022
- 2. 广西医科大学第一附属医院心胸外科，南宁市 530021
- 折叠
摘要
Abstract
Objective To establish a neointima model of pulmonary arterial hypertension (PAH) in rats by pneumonectomy(PE) and monoerotatine(MCT),and to investigate the expression and function of phosphodiesterase 1C (PDE1C) protein in this PAH model.Methods Three approaches were used to establish PAH model in rats:left lung PE group(PE group),MCT injection group,and MCT injection after PE group (P+M group).Mean pulmonary arterial pressure(mPAP),percentage of media thickness(MT%) and the ratio of right ventricular hypertrophy index (RV/LV+S) of model rats were recorded.Morphological parameters relevant to neointima formation and the muscularization of nonmuscle pulmonary arterioles were observed,and the difference of pulmonary vascular remodeling pattern of the three PAH models was analyzed.The expression of PDE1C was detected in each group by immunohistochemitry.Results The P+M group showed serious lesions in the neointim and right ventricular,and serious right ventricular hypertrophy,and the increasing MT of pulmonary arterials could be seen in the animals.Compared with other groups,P+M group had a significantly higher mPAP and muscularization of nonmuscle arterioles developed significantly.Expression of PDE1C protein increased significantly in the neointima PAH model(P<0.05).Conclusion P+M model is a good model for simulation of severe PAH in human , which is the best animal model for a study on the obstructive PAH.Up-regulation of PDE1C in pulmonary artery smooth muscle cells of PAH animal model might offer a new target for therapy of PAH in human .关键词
肺动脉高压/血管重构/新生内膜/磷酸二酯酶/动物模型/大鼠Key words
Pulmonary hypertension/Vascular remodeling/Neointima/Phosphodiesterase/Animal model/Rat分类
医药卫生引用本文复制引用
郑晓宇,覃家锦,李波,何巍,冯旭..大鼠肺动脉高压动物模型的建立及磷酸二酯酶1C的表达[J].广西医学,2013,(4):418-421,4.
