广西医学Issue(10)：1284-1287,4.DOI:10.11675/j.issn.0253-4304.2013.10.04
荧光原位杂交技术对骨髓增生异常综合征7号染色体核型异常的诊断价值
Abnormalities in Chromosome 7 in Myelodysplastic Syndromes Diagnosed by Fluorescence in situ Hybridization
摘要
Abstract
Objective To investigate the significance of fluorescence in situ hybridization ( FISH) applied to the diagnosis of abnormalities in chromosome 7 in myelodysplastic syndromes ( MDS ) .Methods The bone marrow samples collected from 84 MDS patients were analyzed for chromosomal karyotypes by interphase FISH and convention -al cytogenetic analysis(CCA),respectively.A comparison was done for the detection relevance ratio of abnormalities in chromosome 7 between FISH and CCA .Results Among 84 MDS patients,35 with chromosome aberrations were accounted for 41 .7%( 35/84 ) , in which 9 cases of numerical abnormality of chromosomes , accounted for 25 .7%(9/35),were dominated by +8;Thirteen cases of chromosomal structural abnormality ,accounted for 37.1%(13/35),were dominated by 7q-,5q-and 20q-;There were 13 cases of structural and numerical abnormality ,accoun-ted for 37.1%(13/35).The most common chromosomal aberration was abnormality in chromosome +8(11 cases), the follows as complex chromosomal aberrations ( 15 cases ) , abnormality in chromosome 7 ( 9 cases ) , abnormality in chromosome 5q-(5 cases) ,abnormality in chromosome 20q-(5 cases),abnormality in chromosome Y (2 cases). Nine cases of abnormality in chromosome 7,accounted for 10.7%(9/84),were confirmed by FISH,while only 1 case of abnormality in chromosome 7,accounted for 1.2% (1/84),was confirmed by CCA (P <0.01).Conclusion FISH has a higher detection relevance ratio of abnormality in chromosome 7 in MDS patients compared with CCA ,but FISH can′t take the place of CCA .关键词
骨髓增生异常综合征/7号染色体核型异常/荧光原位杂交Key words
Myelodysplastic syndrome/Abnormality in chromosome 7/Fluorescence in situ hybridization分类
医药卫生引用本文复制引用
刘列,刘振芳,罗军,苏良焱,赖永榕..荧光原位杂交技术对骨髓增生异常综合征7号染色体核型异常的诊断价值[J].广西医学,2013,(10):1284-1287,4.基金项目
中华医学会分子生物学临床应用研究专项资金（CAMB042010）；广西自然科学基金 （）
