广西医学2015，Vol.37Issue(8)：1049-1052,4.DOI:10.11675/j.issn.0253-4304.2015.08.01
精氨酸加压素对大鼠心肌成纤维细胞增殖及其胶原合成的影响
Effect of arginine vasopressin on proliferation and collagen synthesis of cardiac fibroblasts in rats
摘要
Abstract
Objective To explore the effect of arginine vasopressin( AVP) on the proliferation and collagen synthesis of cardiac fibroblasts( CFs) .Methods SD rats were given AVP by intravenous injection.Masson staining was performed to assess myocardial fibrosis, and Western Blot was used to detect the expression of transforming growth factor( TGF)-β1.CFs were isolated from neonatal SD rats by collagenase digestion.MTT assay was used to determine the proliferation of CFs,and 3 H-proline incorporation to determine the collagen synthesis of CFs.Results SD rats presented myocardial fibrosis when treated with AVP continuously with the increasing expression of TGF-β1.Both MTT optical density and 3 H-proline incorporation rate of CFs increased gradually with the increasing concentration of AVP. And they were significantly higher than the controls when the concentration of AVP was 10 -6 mol/L(P<0.05).MTT optical density and 3 H-proline incorporation rate of CFs increased gradually with the increasing treatment time based on the intervention of 10 -7 mol/L AVP. And there was significant difference between 36-hour culture group and 6-hour culture group(P<0.05).Conclusion Intervention with high concentration of AVP for a long time might promote the proliferation and collagen synthesis of CFs by improving the expression of myocardial TGF-β1 in rats,thus resulting in myocardial fibrosis.关键词
心肌成纤维细胞/精氨酸加压素/胶原/转化生长因子-β1/大鼠Key words
Cardiacfibroblasts/Argininevasopressin/Collagen/Transforminggrowthfactor-β1/Rat分类
医药卫生引用本文复制引用
张东伟,康艳霞,李娜,刘丽媛,郭艳杰,曹艳杰,范延红..精氨酸加压素对大鼠心肌成纤维细胞增殖及其胶原合成的影响[J].广西医学,2015,37(8):1049-1052,4.基金项目
国家自然科学基金(81370194) （81370194）
