广西医学2015，Vol.37Issue(8)：1065-1068,4.DOI:10.11675/j.issn.0253-4304.2015.08.05
苦参碱对人胆管癌细胞系QBC939增殖及凋亡的影响及其机制
Effect of matrine on prolifera tion and apoptosis of human cholangiocarcinoma cell line QBC939 and its mechanisms
周欢 1雷宇 1梁凯 1郭晴1
作者信息
- 1. 广西医科大学附属肿瘤医院药学部,南宁市 530021
- 折叠
摘要
Abstract
Object ive To explore the effect of matrine on antiproliferation and apoptosis of human cholangiocarcinoma cell line QBC939 in vitro and its mechanisms.Methods The antiproliferation rate of QBC939 cells induced by different concentrations of matrine was detected using MTT assay,the apoptosis rate of QBC939 cells was assessed by Annexin V-FITC/PI staining method,and the mitochondrial membrane potential was analyzed by Rhodamine 123 assay.The expressions of Cyto-c,Bcl-2,BAX and activated caspase-3 proteins were detected by Western Blot.Results The antiproliferation rate of QBC939 cells increased with the increasing concentration of matrine or treatment time(P<00.01),and the antiproliferation effect of matrine on QBC 939 cells showed a doset-ime dependence.The early apoptosis rate,late apoptosis rate and total apoptosis rate increased with the increasing concentration of matrine (P<0.001),and the apoptosis effect of matrine on QBC939 cells showed dose dependence.Rhodamine 123 assay revealed that the proportion of weak fluorescence peak increased with the concentration of matrine increasing(P<0.001).In the groups of concentrations of 0.5,1.0 and 1.5 mg/ml,the expressions of Cyto-c, BAX and activated caspase-3 increased gradually,while the expression of Bcl-2 decreased compared to the controls.Conclusion Matrine has obvious inhibitory effect on the proliferation of QBC939 cells,and can induce the apoptosis of the cells,whose mechanisms may be related to the regulation of the expressions of Bcl-2/BAX proteins in the mitochondrial pathway and the activation of downstream caspase-3.关键词
胆管癌/苦参碱/凋亡/增殖/线粒体/膜电位Key words
Cholangiocarcinoma/Matrine/Apoptosis/Proliferation/Mitochondria/Membrane potential分类
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周欢,雷宇,梁凯,郭晴..苦参碱对人胆管癌细胞系QBC939增殖及凋亡的影响及其机制[J].广西医学,2015,37(8):1065-1068,4.
