广西植物Issue(1)：89-95,101,8.
RACE 法克隆甜菜 NR 基因及生物信息学分析
RACE cloning and bioinformatics analysis of nitrate reductase in Beta vulgaris
摘要
Abstract
Nitrate reductase is an important and limited enzyme in nitrogen metabolism .The functional research of ni‐trate reductase plays a key role in increasing sugar beet (Sb) yields .In this study ,SbNR gene was cloned by RACE and its 3D structure and physiological function were analysed by bioinformatics and expressed in Arabidopsis thaliana to show the gravity .The results were as follows :the full length of SbNR gene was 2760bp ;SbNR was soluble and hydrophobic ;secondary structure analyses showed that SbNR belonged to mixed protein ;SbNR was a new member of NR family ,containing molybdopterin‐binding ,cytochrome‐binding ,FAD‐binding and NAD‐binding domain .It had no signal peptide ,but one transmembrane domain in C terminal ,and the mutants showed later response to the gravity , and which confirmed that NO involved in plant’s gravity .关键词
甜菜/生物信息学/硝酸还原酶/RACE/重力Key words
Beta vulgaris/bioinformatics/nitrate reductase(NR)/rapid amplification of cDNA ends(RACE)/gravity分类
生物科学引用本文复制引用
王琦,冯二艳,王荣,任嘉红..RACE 法克隆甜菜 NR 基因及生物信息学分析[J].广西植物,2013,(1):89-95,101,8.基金项目
国家自然科学基金(31100471) （31100471）
长治学院校级课题(201221) （201221）
