广西植物Issue(6)：792-795,4.DOI:10.11931/guihaia.gxzw201405028
罗汉果皂苷 V 在人工胃液中的稳定性和体外代谢研究
Stability of mogroside V in artificial gastric juice and its metabolism in vitro
摘要
Abstract
In order to evaluate the stability of mogroside V in gastrointestinal tract and study its in vitro metabolism, mogroside V was incubated with artificial gastric juice and human intestinal bacteria,and a liquid chromatography/mass spectrometry method by LCMS/IT-TOF was applied to analyze the products of mogroside V in artificial gastric juice and human intestinal bacteria culture medium.The results indicated that mogroside V was degraded quickly in artificial gas-tric juice to be secondary glycosides with two glycosyls or one glycosyl and mogrol without glycosyl.And the longer time it was incubated,the more completely it was degraded,its product was mogrol while the incubation time was 4 h.While in culture medium of human intestinal bacteria,mogroside V was transformed to mogroside IV(isomer)mogroside III(i-somer),mogroside II(isomer)and mogroside I(isomer)by deglycosylation reaction,as well as to glycoside with six gly-cosyls,by glucosylation reaction.Therefore,a general knowledge on the degradation of mogroside V was obtained after this experiment,which would shed light on the further exploitation of mogroside V and Siraitia grosvenorii .关键词
罗汉果皂苷 V/人肠道菌群/人工胃液/液相色谱-质谱联用Key words
mogroside V/human intestinal bacteria/artificial gastric juice/liquid chromatography/mass spectrometry分类
医药卫生引用本文复制引用
卢凤来,黄振聪,颜小捷,陈月圆,徐风,蔡少青,李典鹏..罗汉果皂苷 V 在人工胃液中的稳定性和体外代谢研究[J].广西植物,2015,(6):792-795,4.基金项目
国家自然科学基金(81160392,81160518) （81160392,81160518）
国家科技支撑计划项目(2011BAI01B03) （2011BAI01B03）
桂林市科学研究与技术开发项目(20120118-6,20110112-16)。 （20120118-6,20110112-16）
