解放军医药杂志2015，Vol.27Issue(9)：7-12,6.DOI:10.3969/j.issn.2095-140X.2015.09.002
DPPC抑制A549细胞增殖、阻滞细胞周期及诱导DNA损伤的初步探究
A Study on A549 Cell Proliferation, Arrested Cell Cycle and Induced DNA Damage Inhibited by DPPC
摘要
Abstract
Objective To discuss the effect of DPPC on anti-proliferation in human non-small cell lung cancer A549 cells, and to investigate the cell cycle arrest and DNA damaged molecular mechanism by DPPC. Methods The A549 cells were cultured in vitro, and morphologic changes of tumor cells were observed using MTT ( thiazolyl blue) as-say after A549 cells were treated with different doses and times. Morphologic changes were observed under inverted mi-croscope after A549 cells were treated with 0. 5μmol/L DPPC. The changes of cell cycle were detected using flow cytom-etry after A549 cells were treated with different does DPPC. Regulatory protein expressions of cyclinB1, cdc2 (p34) and DNA damaged γ-H2AX factor in cell cycle were detected using Western blot test, and the expressions were compared with those by Etoposide treatment and control group ( without treatment) . Results MTT assay showed that tumor cells proliferation obviously inhibited after A549 cells were treated with different concentrations (0. 001-10 μmol/L) for 48 h and same concentration (0. 1 μmol/L) for different times (12-72 h) DPPC, and it had a dose-and time-dependent. In-verted microscope showed that the A549 cells were total atrophy with cell debris, and morphologic changes such as little gas vacuole was found in limbic plasmalemma. The flow cytometry showed that DPPC obviously affected A549 cell cycle to make the blocking cell cycle in G2/M phase. Western blot showed that DPPC could promote cyclinB1 and cdc2 (p34) expressions and inhibit p-cdc2 expression, and enhanced H2AX phosphorylation. Conclusion DPPC may obviously in-hibit proliferation of non-small-cell lung carcinoma cells, block cell cycle in G2/M phase, which possibly relate with up-regulation expression of regulatory protein CyclinB1, phosphorylation inhibition of cdc2(p34) and induction of DNA dam-age. In addition, DPPC shows superior effect of anti-tumor cell proliferation in vitro.关键词
鬼臼毒素/非小细胞肺癌/细胞增殖/细胞周期蛋白类/DNA损伤Key words
Podophyllotoxin/Carcinoma/non-small-cell lung/Cell proliferation/Cyclins/DNA damage分类
医药卫生引用本文复制引用
桑春艳,杨霄鹏,张富婷,李君红,惠玲..DPPC抑制A549细胞增殖、阻滞细胞周期及诱导DNA损伤的初步探究[J].解放军医药杂志,2015,27(9):7-12,6.基金项目
国家自然科学基金资助项目(81372177) （81372177）
全军医药卫生科研基金课题资助项目(CLZLZJB04) （CLZLZJB04）
