解放军医药杂志2015，Vol.27Issue(10)：4-7,4.DOI:10.3969/j.issn.2095-140X.2015.10.002
骨髓间充质干细胞在大鼠脊髓损伤治疗中的应用
Application of Bone Marrow Mesenchymal Stem Cells in Treatment of Rats with Spinal Cord Injuries
摘要
Abstract
Objective To investigate the effect of transplanting bone mesenchymal stem cells ( MSCs) in repair of rats with spinal cord injuries ( SCI) . Methods Bone marrow MSCs were isolated and cultured using whole bone mar-row cells adherence method, and models of SCI were established using free falling body hitting method. A total of 24 rats survived after model establishment, and were divided into transplantation group ( n=12 ) and control group ( n=12 ) . Transplantation group was transplanted with mesenchymal stem cells in injury sites, while control group was treated with same values of physiological saline. The recovery conditions of locomotory capabilities were evaluated using combined praxiology score, and the medullary histopathological changes, injuries and repair of nerve cells and expression of SRY gene were observed. Results The combined praxiology scores of transplantation group at different time points after the treatment were higher than those of control group (P<0. 05). In control group, there were cavities in spinal cord tissues and dead nerve fibers and cells. In transplantation group, the number of cavities was decreased, but hyperplastic tissues was increased uncreased as well as nerve fibers and cells, and SRY gene expression was found. Conclusion Transplan-tation of bone marrow MSCs may improve SCI repair.关键词
骨髓细胞/间质干细胞移植/脊髓损伤/大鼠Key words
Bone marrow cells/Mesenchymal stem cell transplantation/Spinal cord injuries/Rats分类
医药卫生引用本文复制引用
薛荣利,张媛媛,李泽慧,周丹,张晨晨,哈小琴..骨髓间充质干细胞在大鼠脊髓损伤治疗中的应用[J].解放军医药杂志,2015,27(10):4-7,4.基金项目
全军"十二五"面上项目(CWS11C229) （CWS11C229）
