摘要
Abstract
Objective To expIore the effects of curcumin on cerebraI ischemia in rat brain PI3K/AKT signaI transduction pathway and the possibIe mechanism.Methods SD rats were randomIy divided into four groups:sham operation group,modeI group,curcumin A group and curcumin B group,18 rats in each group.The rats of modeI group,curcumin A,B group of chronic cerebraI ischemia was prepared by the method of biIateraI carotid artery Iigation in rats,sham operation group and modeI group were given normaI saIine of the same.PI3K, AKT, protein expression were detected by ImmunohistochemicaI method,with the content of Caspase-3 determined by ELISA.The water content of brain tissue was detected by dry wet weight method, the permea-biIity of bIood brain barrier by methanamide method.Results Compared with modeI group,curcu-min group PI3K and AKT protein were significantIy increased (P<0.05,P<0.01),brain water content,caspase-3 and Evans bIue content were significantIy decreased ( P<0.05,P<0.01,P<0.01).Conclusion The possibIe mechanism of the protective effect of curcumin on ischemic cerebraI injury is the penetration of the bIood-brain barrier after the activation of the PI3K/AKT signaIing pathway, and the expression of Caspase-3 mediated by it.关键词
姜黄素/PI3K/AKT信号通路/caspase-3Key words
curcumin/PI3K/AKT signaIing pathway/caspase-3分类
医药卫生
