华北农学报Issue(2)：60-62,3.
小麦转录因子 TaSIM 的原核表达分析
Prokaryotic Expression Analysis of Transcription Factor TaSIM from Triticum aestivum L.
摘要
Abstract
To investigate the function of TaSIM gene,the cDNA of TaSIM gene was amplified by PCR using pJET1.2-TaSIM as template and the full-length open reading frame was fused into a prokaryotic expression vector pET-28a.PCR and sequencing showed that the recombinant vector pET-28a-TaSIM was successfully constructed and transformed into E.coli BL21 (DE3) cells.The results indicated that the pET-28a-TaSIM with the predicted molecular weight of about 33 kDa was successfully expressed in E.coli BL21 (DE3) strain.SDS-PAGE indicated that the best expression quantity was induced with 0.5 mmol/L IPTG treatment for 2 h at 37 ℃.关键词
小麦/TaSIM/原核表达Key words
Wheat/TaSIM/Prokaryotic expression分类
生物科学引用本文复制引用
于月华,耿洪伟,陈全家,高文伟,王莉萍,曲延英..小麦转录因子 TaSIM 的原核表达分析[J].华北农学报,2013,(2):60-62,3.基金项目
新疆农业大学校前期资助课题（XJAU201019）；新疆维吾尔自治区高技术研究发展项目（201011109）；新疆维吾尔自治区高校科研启动项目（XJEDU2011S20）；作物遗传育种重点学科资助项目 （）
