华北农学报Issue(2):63-69,7.
两个大豆开花期 QTL 定位及对农艺性状的影响分析
Two Quantitative Trait Loci for Flowing Time and Their Effect on Agronomic Traits in Soybean
摘要
Abstract
A cross of Jidou 12 and nf 58 was made to construct a recombinant inbred line of F 9:11 population which has 175 progenies as the experimental material .A genetic linkage map of soybean genome was constructed , which include 117 markers and covering 1 373.6 cM.CIM method of QTL Cartographer v2.5 software and NWIM method of QTL Network 2.0 were used to identify quantitative trait loci and two QTLs were detected .qFTO-1 were located in the marker interval Satt581~Sat_190 of O linkage group,additive value were 2.49 and 2.02,favorable gene from nf 58 and the highest genetic contribution rate is27 .2 %.qFTC2-1 was located in the marker interval Satt557~Sat_251 of C2 linkage group,additive value were -3.50 and-3.84,favorable gene from Jidou 12 and the highest genetic contribution rate is 55.6%.Additive ×Additive epistatic effect was existent between the two QTLs . The value is -0.672 8 and the recombinant type is better than the parent .The two QTL effected on yield related traits obviously and consistent to the result of phenotype analysis .qFTO-1 can increase 2.62 g of yield per plant and qFTC2-1 can increase 2.26 g.These studies provide a theoretical basis for marker-assisted breeding and molecular cloning.关键词
大豆/开花期/相关分析/QTL/上位互作效应Key words
Soybean/Flowering time/Correlation analysis/Quantitative trait loci/Epistatic effect分类
农业科技引用本文复制引用
王涛,杨春燕,赵青松,闫龙,肖付明,智海剑,张孟臣..两个大豆开花期 QTL 定位及对农艺性状的影响分析[J].华北农学报,2013,(2):63-69,7.基金项目
国家“863”计划(2012AA101106);国家自然科学基金(31000719);国家转基因生物新品种培育重大专项(2011ZX08009-088B);国家现代农业产业技术体系 ()