华北农学报Issue(2)：219-224,6.
两株降解菌混合对毒死蜱的降解特性研究
Biodegradation Characteristics of Chlorpyrifos by Mixed Culture of Bacillus cereus Strains
摘要
Abstract
In the present work,we investigated for the first time the degradation characteristics of chlorpyrifos by the mixed culture of Bacillus cereus strains.The optimal ratio of inoculation volume of the two strains for chlor-pyrifos degradation was established .Further,the effects of the inoculation volume,glucose concentration,initial pH and the initial chlorpyrifos concentration on the degradation efficiency of chlorprifos were determined .Simultaneous-ly,the effect of high salinity on the biodegradation activity of chlorpyrifos was also examined .The results showed that when the ratio(V/V) was 1∶1,the degradation rate to 80 mg/Lof chlorpyrifos reached the highest .The optimal in-oculum amount of the mixed strains was 8%(V/V) of the total volume.Addition of glucose stimulated the growth of the mixed strains but did not enhance the degradation efficiency of chlorpyrifos .The alkaline environment was prone to form the biomass and to achieve a high degradation rate of chlorpyrifos by the mixed strains .When the initial con-centration of chlorpyrifos was 42 mg/L,the residual concentration of chlorpyrifos decreased with the time .However, the degradation curves of chlorpyrifos at high concentrations (108,126 mg/L) followed two peaks.The degradation rate of the mixed strains to 80 mg/L of chlorpyrifos was above 41%while the sodium chloride concentration ranged from 20 g/L to 70 g/L.The results suggest that the mixed strains could be used as a potential and efficient chlor -pyrifos degrader for the bioremediation of contaminated sites ,especially for the cleanup of high salinity wastewater in the chlorpyrifos production enterprises .关键词
毒死蜱/蜡状芽孢杆菌/生物降解/混合菌/高盐Key words
Chlorpyrifos/Bacillus cereus/Biodegradation/Mixed strains/High salinity分类
资源环境引用本文复制引用
段海明..两株降解菌混合对毒死蜱的降解特性研究[J].华北农学报,2013,(2):219-224,6.基金项目
国家高技术研究发展计划项目（2007AA10Z404）；安徽科技学院预研项目 （）
