华北农学报Issue(3)：25-29,5.
大豆异黄酮生物合成途径中 IFS1基因表达分析
Expression Analysis of IFS1 Gene in Isoflavonoids Pathway in Soybean
摘要
Abstract
Previous studies have shown that isoflavone synthase (IFS) encode key enzymes in the isoflavonoids pathway in Soybean.There are two IFS homologues IFS1 and IFS2 in Soybean,The expression level of IFS1 gene in different tissues will be quantified by fluorescence quantitative PCR .The results revealed that the transcripts were widely distributed in all the tested tissues .IFS1 gene showed the higher expression level in the tender organs :root, stem,leaf germinating 7 days than that in the older organs :roots,stems,leaves growing 70 days.Expression level in seeds at 25 days and 45 days post anthesis were also lower ,while with higher content in the mature embryo ,sugges-ting that IFS1 gene has finished the Soybean isoflavone accumulation process in the early embryo formation .Fur-ther,the analysis of expression induced by abiotic treatments indicated that the genes were differentially regulated under IAA,ABA,PEG,NaCl,cold and wounding treatments .It will provide more knowledges on isoflavone biosyn -thesis and resistance to abiotic stress in Soybean .关键词
大豆/异黄酮/异黄酮合酶/基因表达Key words
Soybean/Isoflavones/Isoflavone synthase/Gene expression分类
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练云,李海朝,王树峰,武永康,李金英,雷晨芳,卢为国..大豆异黄酮生物合成途径中 IFS1基因表达分析[J].华北农学报,2013,(3):25-29,5.基金项目
转基因生物新品种培育重大专项（2011ZX08004－003）；国家“863”项目 （）
