华北农学报Issue(3)：77-81,5.
小麦品系 L224－3抗叶锈病基因的分子作图
Molecular Mapping of Leaf Rust Resistance Genes in Wheat Line L224-3
摘要
Abstract
Wheat line L224-3 showed high resistance to most of Puccinia triticina pathotypes in the seedling and adult stage in the field.Identify and map the leaf rust resistance gene (s) in L224-3 is very useful to breed wheat cultivars with durable resistance to leaf rust .L224-3 and 37 check lines with known leaf rust resistance genes were inoculated with 15 pathotypes at seedling stage to postulate gene in L224-3.F2:3 population from the cross L224-3 /Zhengzhou 5389 were identified with pathotype FHDQ for genetic analysis of leaf rust resistance in L 224-3. SSR and STS markers were used to map the gene in L224-3.L224-3 might contain a dominant resistance gene ,tem-porarily designated LrL224.Totally four SSR markers (barc8,gwm582,wmc419,wmc694) and one STS marker (ω-secali /Glu-B3) on 1B were closely linked to LrL224.The two flanking SSR loci were barc8 and gwm582,with the genetic distances of 4.3 and 4.6 cM,respectively.LrL224 was located on 1BL,and it showed different seedling re -action with other genes on 1B.Therefore LrL224 is likely to be a new leaf rust resistance gene .关键词
小麦/抗叶锈病基因/遗传分析/分子标记/分子作图Key words
Wheat/Leaf rust resistance gene/Gene postulation/Genetic analysis/Molecular mapping分类
农业科技引用本文复制引用
李欢,郑嫚嫚,王翠芬,范林林,李亚宁,李在峰,刘大群..小麦品系 L224－3抗叶锈病基因的分子作图[J].华北农学报,2013,(3):77-81,5.基金项目
国家自然科学基金项目 （）
