华北农学报Issue(4)：7-12,6.
大豆中2个编码 PR10蛋白新基因的克隆与分析
Cloning and Analysis of Two New PR10 Members in Soybean
摘要
Abstract
Two new soybean genes named Glyma09g04530 and Glyma15g15590 which encoding PR10 proteins were cloned from a SMV resistant cultivar Kefeng No .1.Glyma09g04530 and Glyma15g15590 have a 477 bp ORF and coding 158 amino acids .Sequence alignment and evolutionary tree clustering analysis showed that Gly-ma09g04530 and Glyma15g15590 are new PR10 protein genes in soybean .The genes of Glyma09g04530 and Gly-ma15 g15590 expressed in root ,stem and leave by using Real-time PCR analysis in soybean .Expression level of Gly-ma09g04530 and Glyma15g15590 were strongly induced and highly expressed in leaves of Kefeng No .1 after inocu-lation SMV .We speculate that Glyma09 g04530 and Glyma15 g15590 may be involved for getting resistance to SMV .关键词
大豆/PR10蛋白/基因克隆/表达分析Key words
Soybean/PR10 protien/Gene cloning/Expression analysis分类
生物科学引用本文复制引用
陈华涛,陈新,顾和平,张红梅,袁星星,崔晓艳..大豆中2个编码 PR10蛋白新基因的克隆与分析[J].华北农学报,2013,(4):7-12,6.基金项目
江苏省农业科技自主创新项目CX11（1029）；江苏省科技支撑项目（BE2011305）；大豆转基因专项子课题 （）
