华北农学报Issue(4)：37-40,4.
一个 T-DNA 插入突变体的分子鉴定及表达分析
Molecular Identification and Expression Analysis of a T-DNA Insertional Mutant in Rice
摘要
Abstract
Mutants play an important role in rice functional genome research .A T-DNA insertion line was ana-lyzed and identified in this study .The results showed that the T-DNA line was a homozygous insertion of OsFCA genes,and the heading-date of T-DNA insertion mutant was later than that of wild type .The RT-PCR results showed that the T-DNA insertion line was a loss-of-function mutant of OsFCA genes.In addition,the expression analysis found that Hd1 and Hd3 a displayed higher expression levels in wild type in the short day condition than that of the mutant,especially in the dark time,indicating that OsFCA can positively regulate the expression of gene Hd1 and Hd3a under short-day condition.关键词
水稻/T-DNA插入突变/抽穗期/OsFCAKey words
Rice/T-DNA insertion mutagenesis/Heading date/OsFCA分类
生物科学引用本文复制引用
仪治本,梁小红..一个 T-DNA 插入突变体的分子鉴定及表达分析[J].华北农学报,2013,(4):37-40,4.基金项目
国家转基因重大专项 （）
