华北农学报Issue(4)：41-45,5.
植物病原菌诱导表达载体构建及在烟草中的瞬时表达研究
Construction of a Plant Inducible Expression Vector and Its Transient Expression in Tobacco
摘要
Abstract
According to the sequences of pathogen-inducible plant promoters PPP3 at GenBank,PPP3 promot-ers was cloned from tobacco genome .It was used to replace cauliflower mosaic virus 35 S promoter of pCAM-BIA1301 .The recombinant plasmid was used to transform Agrobacterium tumefaciens GV3101 .The inducibility of the PPP3 promoters in tobacco leaf was evaluated by Agrobacterium tumefaciens-transient genetic transformation as-sye .Real-time quantitative PCR was used to screen the PPP 3 promoters with high inducible expression .The results showed that the gus transcript level under the control of PPP 3 promoters increased respectively 27 .94 fold and 17.69 fold after inoculation with Ralstonia solanacearum and SA.The PPP3 promoter had the advantages such as low basal activity and high expression activity .关键词
诱导型启动子/瞬时表达/定量PCRKey words
Inducible expression Promoter/Transient expression/Real-time quantitative PCR分类
生物科学引用本文复制引用
欧阳乐军,黄真池,沙月娥,曾富华..植物病原菌诱导表达载体构建及在烟草中的瞬时表达研究[J].华北农学报,2013,(4):41-45,5.基金项目
国家“十二五”科技计划项目（2012BAD19B08）；国家星火计划项目（2011GA780067；2011GA780057）；广东高校边缘热带特色植物资源工程技术开发中心项目（ GCZX-B1002）；广东省自然科学基金项目（ S2012010008737；10152404801000005；10452404801004328）；广东省科技计划项目（2012A020602108）；湛江市科技攻关项目 （）
