华北农学报Issue(4)：46-52,7.
郑丰5号α-醇溶蛋白基因的克隆与序列分析
Cloning and Sequencing of α-gliadin Genes from Common Wheat Cultivar Zhengfeng 5
摘要
Abstract
The α-gliadins are the important components of seed strorage proteins , and their composition and content play a major role in determining the processing quality of wheat .Using PCR technology ,total 32 novel genes ( Designated as ZF5A-1 -ZF5A-32;GenBank No:JX828280 -JX828311 ) were cloned from the wheat cultivar Zhengfeng 5,including 17 full-ORFα-gliadin genes(Designated as ZF5A-1-ZF5A-17) and 15 pseudogenes.The sequence analysis of deduced amino acids showed that 10 had the typical structural characteristics of α-gliadin genes reported previously,and 7(ZF5A-1,ZF5A-3,ZF5A-6,ZF5A-9,ZF5A-10,ZF5A-11,ZF5A-15)possessed an additional cysteine residue in the unique domain Ⅱ.Based on the occurrence of the four major T cell immunogenic peptides and glutamine residues in the glutamine repeats ,ZF5A-7 and ZF5A-12 could be assigned to chromosome 6A,ZF5A-4,ZF5A-13,ZF5A-14 and ZF5A-17 to chromosome 6B,and other 11 genes to chromosome 6D.The sec-ondary structure predication of 17 cloned genes and other four genes in public database demonstrated that the num-bers and distributions of α-helix and β-strand were variable in different mature α-gliadins.Generally,5 α-helixes ( H1 -H5 ) usually presented in the two glutamine repeats and two unique domains for majority of α-gliadins,but the putative proteins of ZF5A-17 lost the α-helix(H2),ZF5A-2 and ZF5A-8 had an additional α-helix in the unique domain Ⅰ(HE1),and GQ891685 and ZF5A-15 had an additional α-helix in the second glutamine repeat(HE2).In addition,most of the genes(61.11%)had a β-strand(E)in the C-terminal unique domain Ⅱ.High variations in numbers of cysteine residue ,α-helixes and β-strands in the α-gliadins of Zhengfeng 5 might be strongly related to its good rheological properties of dough .关键词
α-醇溶蛋白/郑丰5号/克隆/序列分析Key words
α-gliadin/Zhengfeng 5/Gene clonging/Sequence analysis分类
农业科技引用本文复制引用
李玉阁,邢冉冉,崔一飞,李锁平..郑丰5号α-醇溶蛋白基因的克隆与序列分析[J].华北农学报,2013,(4):46-52,7.基金项目
“十二五”农村领域国家科技计划项目（2011BAD07B00；2012AA101105）；自然科学基金面上项目 （）
