华北农学报Issue(4)：228-233,6.
副猪嗜血杆菌分离株的耐药性及耐药基因分析
Phenotype and Genotype Analysis of Antibiotic Resistance of Haemophilus parasuis Isolated from the Scaled Pig Farms in Zhejiang Province
摘要
Abstract
This study was conducted to investigate the resistance to common antibiotics and genes of Haemophi-lus parasuis isolated from the scaled pig farms in Zhejiang province .A total of 73 Zhejiang H.parasuis isolates were tested for their resistance to 18 antibiotics by K-B method.PCR was used to detect related genes .The amplified products were sequenced and aligned .The results showed that 86 .30% isolates were resistant to sulfonamides , 65.75%,60.27%,50.68%,50.68%and 46.58%isolates were resistant to tetracyclines ,aminoglycosides,quino-lones,β-lactam antibiotics,chloramphenicols,respectively,while only 28.77%isolates were resistant to macrolides. Resistance genes were detected in 97.26%isolates,and multiple resistance genes were found in 84.93%isolates. The rates of resistance genes Aac(6′)-1b,AadA1,Sul1,Sul2,Flor,CmLA,Cat1,TetA,TetB,Tem-11 and ErmB were 76.71%,65.75%,45.2%,10.96%,15.07%,8.22%,58.9%,4.11%,49.32%,35.62%and 1.37%,respec-tively.The coincidence of phenotype and genotype for the isolates to resist sulfonamides ,β-lactam antibiotics,chlor-amphenicols, tetracyclines, macrolides, quinolones and aminoglycoside antibiotics were 62.07%, 74.29%, 81.48%,75.00%,0,75.68%and 72.09%,respectively.Therefore,H.parasuis has developed resistance to clini-cally commonly used antibiotics and the results from analysis of drug-resistant gene could be used as reference to determine resistance bacteria .关键词
副猪嗜血杆菌/耐药性/耐药基因Key words
Haemophilus parasuis/Drug resistance/Drug-resistant gene分类
农业科技引用本文复制引用
忽占利,李军星,胡松华,王一成,袁秀芳,徐丽华..副猪嗜血杆菌分离株的耐药性及耐药基因分析[J].华北农学报,2013,(4):228-233,6.基金项目
浙江省自然科学基金项目 （）
